recombinant amph1  (Novus Biologicals)

Journal: Frontiers in Neuroscience

Article Title: Novel autoimmune response in a tauopathy mouse model

doi: 10.3389/fnins.2013.00277

Figure Lengend Snippet: Auto-AMPH1 antibodies are biomarkers of tau-mediated neurodegeneration in the JNPL3 tauopathy mouse model. (A) ELISA shows increased levels of auto-AMPH1 antibodies in JNPL3 mice with motor impairment when compared to normal JNPL3 mice and NTg littermates. Bar graph shows the mean ± standard error of the mean (s.e.m.) for each group. Means were compared by two-way ANOVA; p ** = 0.0032, p * = 0.0102. (B,C) The amount of auto-AMPH1 antibodies in serum positively correlates with motor decline (B) and AMPH1 protein depletion in the CNS (C) , as indicated by the Pearson's “ r ” correlation analysis. The linear regression of the best fit line shows the 95% confidence band; R 2 = 0.3031 for (B) and R 2 = 0.1745 for (C) . (D) ELISA shows increased levels of auto-AMPH1 antibodies in old JNPL3 mice (8.1–13 months of age) when compared to NTg littermates. Bar graph shows the mean ± standard error of the mean (s.e.m.) for each group. Means were compared by paired two-tailed t -test; p * = 0.0378.

Article Snippet: Briefly, the wells of 96-well microplates (for Figure was used BD Biosciences, cat. no. 353228; for Figure was used Fisher Scientific, cat. no. 12565501) were coated with 300 ng of recombinant AMPH1 (Novus Biologicals, cat. no. H00000273-P01) overnight at 4°C.

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Frontiers in Neuroscience

Article Title: Novel autoimmune response in a tauopathy mouse model

doi: 10.3389/fnins.2013.00277

Figure Lengend Snippet: AMPH1 protein depletion is associated with pathological events in tau-mediated neurodegeneration. (A) When compared to NTg controls, JNPL3 mice with impaired motor function show reduced levels of the protein AMPH1 in the spinal cord, as illustrated by immunoblots. The protein level reduction is accompanied by the accumulation of 64kDa hyperphosphorylated hTauP301L. Arrow indicates tau positive bands with a 63.2 kDa molecular weight according to linear semi-logarithmic interpolations. GAPDH chemiluminescence was used as loading control. (B) Densitometry analysis of (A) . Bar graph shows the mean ± s.e.m. for each group. Means were compared by two-way ANOVA ( p *** = 0.0007). (C,D) AMPH1 protein levels in the spinal cord negatively correlate with motor impairment (C) and the accumulation of 64kDa hTauP301L (D) . The linear regression of the best fit line shows the 95% confidence band; R 2 = 0.3597 for (C) and R 2 = 0.3435 for (D) .

Article Snippet: Briefly, the wells of 96-well microplates (for Figure was used BD Biosciences, cat. no. 353228; for Figure was used Fisher Scientific, cat. no. 12565501) were coated with 300 ng of recombinant AMPH1 (Novus Biologicals, cat. no. H00000273-P01) overnight at 4°C.

Techniques: Western Blot, Molecular Weight, Control

Journal: Frontiers in Neuroscience

Article Title: Novel autoimmune response in a tauopathy mouse model

doi: 10.3389/fnins.2013.00277

Figure Lengend Snippet: AMPH1 protein level decrease in the CNS is accompanied by increased levels of auto-AMPH1 antibodies in a JNPL3 mouse . (A) The abundance of AMPH1protein is significantly reduced in the spinal cord of a JNPL3 mouse in comparison to a non-transgenic (NTg) littermate. (B) Serum from a JNPL3 mouse with motor impairment and a normal NTg littermate were collected and used to recognize recombinant AMPH1 (Ponceau). Blots show increased levels of auto-AMPH1 antibodies in the serum of the JNPL3 mouse that showed AMPH1 protein level reduction in the spinal cord, but not the serum derived from the NTg mouse.

Article Snippet: Briefly, the wells of 96-well microplates (for Figure was used BD Biosciences, cat. no. 353228; for Figure was used Fisher Scientific, cat. no. 12565501) were coated with 300 ng of recombinant AMPH1 (Novus Biologicals, cat. no. H00000273-P01) overnight at 4°C.

Techniques: Comparison, Transgenic Assay, Recombinant, Derivative Assay